Intermediate

Part:BBa_K1613024:Experience

Designed by: Lee, Chang-Lung   Group: iGEM15_HSNU-TAIPEI   (2015-09-14)

Whether lead can enter e.coli or not: By using gold nanoparticles(AU-NPs), we measured the lead absorption of e.coli. The results showed that the longer e.coli put in Pb2+ or the higher Pb2+ concentration, the more lead entered the e.coli.

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Applications of BBa_K1613024

Here are our product design: 1. The tube is divided into the left side (red) and the right side (blue) by a partition. The red side is a straw for effectiveness test. Press the red straw so that the test copper ion is added into microcapsules. Green fluorescence means the E.coli survives; if not, we can know that the product is unavailable. The blue side is a tube for piercing. Press the blue straw to pierce chitosan so that the oil can go into the layer of microcapsules. 2. Microcapsule (micro-powder-like): Microcapsule is the most important part of this product, which is made by freeze drying so that the water is reduced. As long as it exposes to any substances such as heavy metals, benzopyrene and aflatoxin, green fluorescence will soon express and from that we know there are toxins in the edible oil. Without tough inspection process, we can easily test the oil samples with very low cost. 3. Chitosan: both layers of chitosan are exceedingly easy to dissolve in acetic acid, which has a big effect in attach to cooper(II) ion. 4. The bottom layer of the tube is sodium percarbonate, an oxidizing agent. Sodium percarbonate is an ingredient in a number of home and laundry cleaning products, including non-chlorine bleach products such as OxiClean, Tide laundry detergent, and Vanish. Dissolved in water, it yields a mixture of hydrogen peroxide (which eventually decomposes to water and oxygen) and sodium carbonate ("soda ash").

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